Rooting and hardening of<i style=""> in vitro</i> plantlets of <i style="">Garcinia</i> <i style="">indica</i> Chois

409-413In the present paper, a procedure for rooting and acclimatization of in vitro plantlets raised from immature seed cultures of Garcinia indica is described. Pulse treatment of 9800µM IBA was preferred for rooting of in vitro shoots. Nearly 100% rooting (in vitro) was obtained with half strength MS with 2% sucrose and 0.6 % agar. Rooted plantlets of about 2-4 cm height (2-3 nodes) were subsequently transferred to different media for acclimatization: 1. Cocopeat + Sand + Soil (1:2:1); 2. Cocopeat + Sand (1:1); 3. Cocopeat. Plants grown in cocopeat showed 76% of survival followed by cocopeat and sand (1:1) with 75% of survival. The in vitro elongated shoots could also be rooted ex vitro by treating with synthetic hormones like Keradix, Rootex-1, and Rootex-3. However, the success of rooting was low (20 to 55 %). The hardened plantlets were successfully acclimatized in the greenhouse and transferred to open field conditions